Details of assembly methods and in silico genotyping for Clostridioides
All MLST-like typing methods in EnteroBase are derived from a genome assembly of sequenced reads. For an explanation of this method, see here
For a general description of the in silico typing method, see here
MLST – Classic |
Ribosomal MLST (Jolley, 2012) |
Core Genome MLST |
Whole Genome MLST |
|---|---|---|---|
7 Loci |
53 Loci |
2,556 Loci |
11,490 Loci |
Conserved Housekeeping genes |
Ribosomal proteins |
Core genes |
Any coding sequence |
Highly conserved; Low resolution |
Highly conserved; Medium resolution |
Variable; High resolution |
Highly variable; Extreme resolution |
Different scheme for each species/genus |
Single scheme across tree of life |
Different scheme for each species/genus |
Different scheme for each species/genus |
7 Gene MLST
The classic MLST scheme for Clostridioides is described in Griffiths et al (2010) J. Clin. Microbiol. 48, 770-778.
Genes included in 7 gene MLST (together with the length of sequence used for MLST taken from table 1 in the above cited paper):
Gene |
Length |
|---|---|
adk |
501 |
atpA |
555 |
dxr |
411 |
glyA |
516 |
recA |
564 |
sodA |
450 |
tpi |
504 |
Ribosomal MLST (rMLST)
rMLST is Copyright © 2010-2016, University of Oxford. rMLST is described in Jolley et al. 2012 Microbiology 158:1005-15.