Details of assembly methods and in silico genotyping for Clostridioides

  • All MLST-like typing methods in EnteroBase are derived from a genome assembly of sequenced reads. For an explanation of this method, see here

  • For a general description of the in silico typing method, see here

MLST – Classic

Ribosomal MLST (Jolley, 2012)

Core Genome MLST

Whole Genome MLST

7 Loci

53 Loci

2,556 Loci

11,490 Loci

Conserved Housekeeping genes

Ribosomal proteins

Core genes

Any coding sequence

Highly conserved; Low resolution

Highly conserved; Medium resolution

Variable; High resolution

Highly variable; Extreme resolution

Different scheme for each species/genus

Single scheme across tree of life

Different scheme for each species/genus

Different scheme for each species/genus

7 Gene MLST

The classic MLST scheme for Clostridioides is described in Griffiths et al (2010) J. Clin. Microbiol. 48, 770-778.

Genes included in 7 gene MLST (together with the length of sequence used for MLST taken from table 1 in the above cited paper):

Gene

Length

adk

501

atpA

555

dxr

411

glyA

516

recA

564

sodA

450

tpi

504

Ribosomal MLST (rMLST)